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Oxygen and glucose deprivation The SVGp12 normal human astrocyte cell line (American Type Culture Collection, ATCC, USA) was cultured in Dulbeccos modified Eagles medium (DMEM, HyClone, USA) containing 10% fetal bovine serum (FBS, Gibco, USA) at 37 C with 5% CO 2

But sometimes, you might need a little bit more to really get all of the old protein out of there

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Around 13 mL per vial is common